Structural bias in T4 RNA ligase-mediated 3′-adapter ligation

T4 RNA ligases are commonly used to attach adapters to RNAs, but large differences in ligation efficiency make detection and quantitation problematic. We developed a ligation selection strategy using random RNAs in combination with high-throughput sequencing to gain insight into the differences in e...

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Detalles Bibliográficos
Autores principales: Zhuang, Fanglei, Fuchs, Ryan T., Sun, Zhiyi, Zheng, Yu, Robb, G. Brett
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2012
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3326334/
https://www.ncbi.nlm.nih.gov/pubmed/22241775
http://dx.doi.org/10.1093/nar/gkr1263

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3326334/
https://www.ncbi.nlm.nih.gov/pubmed/22241775
http://dx.doi.org/10.1093/nar/gkr1263

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