An easy and versatile 2-step protocol for targeted modification and subcloning of DNA from bacterial artificial chromosomes using non-commercial plasmids

BACKGROUND: Promoter-specific expression of foreign DNA in transgenic organisms often relies on bacterial artificial chromosomes (BACs). This approach requires modification and subcloning of BAC-DNA by recombineering technologies in Escherichia coli. Most current protocols rely on commercial kits or...

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Detalles Bibliográficos
Autores principales: Hartwich, Heiner, Nothwang, Hans Gerd
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Technical Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3327625/
https://www.ncbi.nlm.nih.gov/pubmed/22433714
http://dx.doi.org/10.1186/1756-0500-5-156

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3327625/
https://www.ncbi.nlm.nih.gov/pubmed/22433714
http://dx.doi.org/10.1186/1756-0500-5-156

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