Integration of DNA into bacterial chromosomes from plasmids without a counter-selection marker

Most bacteria can only be transformed with circular plasmids, so robust DNA integration methods for these rely upon selection of single-crossover clones followed by counter-selection of double-crossover clones. To overcome the limited availability of heterologous counter-selection markers, here we e...

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Detalles Bibliográficos
Autores principales: Heap, John T., Ehsaan, Muhammad, Cooksley, Clare M., Ng, Yen-Kuan, Cartman, Stephen T., Winzer, Klaus, Minton, Nigel P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2012
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3333862/
https://www.ncbi.nlm.nih.gov/pubmed/22259038
http://dx.doi.org/10.1093/nar/gkr1321

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3333862/
https://www.ncbi.nlm.nih.gov/pubmed/22259038
http://dx.doi.org/10.1093/nar/gkr1321

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