Flp and Cre expressed from Flp–2A–Cre and Flp–IRES–Cre transcription units mediate the highest level of dual recombinase-mediated cassette exchange

Recombinase-mediated cassette exchange (RMCE) is a powerful tool for unidirectional integration of DNA fragments of interest into a pre-determined genome locale. In this report, we examined how the efficiency of dual RMCE catalyzed by Flp and Cre depends on the nature of transcription units that exp...

Descripción completa

Detalles Bibliográficos
Autores principales: Anderson, Rachelle P., Voziyanova, Eugenia, Voziyanov, Yuri
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2012
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3333864/
https://www.ncbi.nlm.nih.gov/pubmed/22270085
http://dx.doi.org/10.1093/nar/gks027

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3333864/
https://www.ncbi.nlm.nih.gov/pubmed/22270085
http://dx.doi.org/10.1093/nar/gks027

Ejemplares similares