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Cell Cycle-Dependent Mobility of Cdc45 Determined in vivo by Fluorescence Correlation Spectroscopy

Eukaryotic DNA replication is a dynamic process requiring the co-operation of specific replication proteins. We measured the mobility of eGFP-Cdc45 by Fluorescence Correlation Spectroscopy (FCS) in vivo in asynchronous cells and in cells synchronized at the G1/S transition and during S phase. Our da...

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Detalles Bibliográficos
Autores principales: Broderick, Ronan, Ramadurai, Sivaramakrishnan, Tóth, Katalin, Togashi, Denisio M., Ryder, Alan G., Langowski, Jörg, Nasheuer, Heinz Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3334904/
https://www.ncbi.nlm.nih.gov/pubmed/22536402
http://dx.doi.org/10.1371/journal.pone.0035537