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Cell Cycle-Dependent Mobility of Cdc45 Determined in vivo by Fluorescence Correlation Spectroscopy
Eukaryotic DNA replication is a dynamic process requiring the co-operation of specific replication proteins. We measured the mobility of eGFP-Cdc45 by Fluorescence Correlation Spectroscopy (FCS) in vivo in asynchronous cells and in cells synchronized at the G1/S transition and during S phase. Our da...
Autores principales: | Broderick, Ronan, Ramadurai, Sivaramakrishnan, Tóth, Katalin, Togashi, Denisio M., Ryder, Alan G., Langowski, Jörg, Nasheuer, Heinz Peter |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3334904/ https://www.ncbi.nlm.nih.gov/pubmed/22536402 http://dx.doi.org/10.1371/journal.pone.0035537 |
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