Development of a Tetrameric Streptavidin Mutein with Reversible Biotin Binding Capability: Engineering a Mobile Loop as an Exit Door for Biotin

A novel form of tetrameric streptavidin has been engineered to have reversible biotin binding capability. In wild-type streptavidin, loop(3–4) functions as a lid for the entry and exit of biotin. When biotin is bound, interactions between biotin and key residues in loop(3–4) keep this lid in the clo...

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Detalles Bibliográficos
Autores principales: O'Sullivan, Valerie J., Barrette-Ng, Isabelle, Hommema, Eric, Hermanson, Greg T., Schofield, Mark, Wu, Sau-Ching, Honetschlaeger, Claudia, Ng, Kenneth K.-S., Wong, Sui-Lam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3334968/
https://www.ncbi.nlm.nih.gov/pubmed/22536357
http://dx.doi.org/10.1371/journal.pone.0035203

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3334968/
https://www.ncbi.nlm.nih.gov/pubmed/22536357
http://dx.doi.org/10.1371/journal.pone.0035203

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