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Development of a Tetrameric Streptavidin Mutein with Reversible Biotin Binding Capability: Engineering a Mobile Loop as an Exit Door for Biotin
A novel form of tetrameric streptavidin has been engineered to have reversible biotin binding capability. In wild-type streptavidin, loop(3–4) functions as a lid for the entry and exit of biotin. When biotin is bound, interactions between biotin and key residues in loop(3–4) keep this lid in the clo...
| Autores principales: | , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2012
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3334968/ https://www.ncbi.nlm.nih.gov/pubmed/22536357 http://dx.doi.org/10.1371/journal.pone.0035203 |