Cargando…

Efficient Reverse Transcription Using Locked Nucleic Acid Nucleotides towards the Evolution of Nuclease Resistant RNA Aptamers

BACKGROUND: Modified nucleotides are increasingly being utilized in the de novo selection of aptamers for enhancing their drug-like character and abolishing the need for time consuming trial-and-error based post-selection modifications. Locked nucleic acid (LNA) is one of the most prominent and succ...

Descripción completa

Detalles Bibliográficos
Autores principales:	Crouzier, Lucile, Dubois, Camille, Edwards, Stacey L., Lauridsen, Lasse H., Wengel, Jesper, Veedu, Rakesh N.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2012
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3338489/ https://www.ncbi.nlm.nih.gov/pubmed/22558297 http://dx.doi.org/10.1371/journal.pone.0035990

Ejemplares similares

G-rich VEGF aptamer with locked and unlocked nucleic acid modifications exhibits a unique G-quadruplex fold
por: Marušič, Maja, et al.
Publicado: (2013)

Rapid One-Step Selection Method for Generating Nucleic Acid Aptamers: Development of a DNA Aptamer against α-Bungarotoxin
por: Lauridsen, Lasse H., et al.
Publicado: (2012)

Enzymatic synthesis of DNA strands containing α-L-LNA (α-L-configured locked nucleic acid) thymine nucleotides
por: Højland, Torben, et al.
Publicado: (2012)

Alpha-l-Locked Nucleic Acid-Modified Antisense Oligonucleotides Induce Efficient Splice Modulation In Vitro
por: Raguraman, Prithi, et al.
Publicado: (2020)

Computational Investigation of Locked Nucleic Acid (LNA) Nucleotides in the Active Sites of DNA Polymerases by Molecular Docking Simulations
por: Poongavanam, Vasanthanathan, et al.
Publicado: (2014)

In vitro evolution of chemically-modified nucleic acid aptamers: Pros and cons, and comprehensive selection strategies
por: Lipi, Farhana, et al.
Publicado: (2016)

Radiolabeling of Nucleic Acid Aptamers for Highly Sensitive Disease-Specific Molecular Imaging
por: Hassanzadeh, Leila, et al.
Publicado: (2018)

Thermodynamics of RNA duplexes modified with unlocked nucleic acid nucleotides
por: Pasternak, Anna, et al.
Publicado: (2010)

Locked nucleic acid (LNA): High affinity targeting of RNA for diagnostics and therapeutics
por: Kauppinen, Sakari, et al.
Publicado: (2005)

Unlocked nucleic acid modified primer-based enzymatic polymerization assay: towards allele-specific genotype detection of human platelet antigens
por: Le, Bao T., et al.
Publicado: (2018)

Development of an Efficient G‐Quadruplex‐Stabilised Thrombin‐Binding Aptamer Containing a Three‐Carbon Spacer Molecule
por: Aaldering, Lukas J., et al.
Publicado: (2017)

Improved thrombin binding aptamer by incorporation of a single unlocked nucleic acid monomer
por: Pasternak, Anna, et al.
Publicado: (2011)

Locked Nucleic Acid: High-Affinity Targeting of Complementary RNA for RNomics
por: Kauppinen, S., et al.
Publicado: (2006)

Labelling of live cells using fluorescent aptamers: binding reversal with DNA nucleases
por: Terazono, Hideyuki, et al.
Publicado: (2010)

Rational Design of Short Locked Nucleic Acid-Modified 2′-O-Methyl Antisense Oligonucleotides for Efficient Exon-Skipping In Vitro
por: Le, Bao T., et al.
Publicado: (2017)

Hybridization-Based Detection of Helicobacter pylori at Human Body Temperature Using Advanced Locked Nucleic Acid (LNA) Probes
por: Fontenete, Sílvia, et al.
Publicado: (2013)

Targeting miR-9 in gastric cancer cells using locked nucleic acid oligonucleotides
por: Lima, Joana Filipa, et al.
Publicado: (2018)

Development of nucleic acid aptamer-based lateral flow assays: A robust platform for cost-effective point-of-care diagnosis
por: Wang, Tao, et al.
Publicado: (2021)

Aptamer-engineered (nano)materials for theranostic applications
por: Rabiee, Navid, et al.
Publicado: (2023)

The ability of locked nucleic acid oligonucleotides to pre-structure the double helix: A molecular simulation and binding study
por: Xu, You, et al.
Publicado: (2019)

Structural transformation induced by locked nucleic acid or 2′–O-methyl nucleic acid site-specific modifications on thrombin binding aptamer
por: Liu, Bo, et al.
Publicado: (2014)

Amplification and Re-Generation of LNA-Modified Libraries
por: Doessing, Holger, et al.
Publicado: (2012)

Improved Cancer Targeting by Multimerizing Aptamers on Nanoscaffolds
por: Omer, Marjan, et al.
Publicado: (2020)

Intercalator conjugates of pyrimidine locked nucleic acid-modified triplex-forming oligonucleotides: improving DNA binding properties and reaching cellular activities
por: Brunet, Erika, et al.
Publicado: (2005)

Intramuscular Evaluation of Chimeric Locked Nucleic Acid/2′OMethyl-Modified Antisense Oligonucleotides for Targeted Exon 23 Skipping in Mdx Mice
por: Georgiadou, Michaella, et al.
Publicado: (2021)

Nucleic Acid Aptamers in Nanotechnology
por: Sinitsyna, Valentina V., et al.
Publicado: (2022)

Effect of Locked-Nucleic Acid on a Biologically Active G-Quadruplex. A Structure-Activity Relationship of the Thrombin Aptamer
por: Bonifacio, Laura, et al.
Publicado: (2008)

Development of bis-locked nucleic acid (bisLNA) oligonucleotides for efficient invasion of supercoiled duplex DNA
por: Moreno, Pedro M. D., et al.
Publicado: (2013)

Modulation of ascites tumour growth by nucleic acids and nucleases.
por: Burdon, R. H., et al.
Publicado: (1982)

Nucleic Acid-Based Theranostics for Tackling Alzheimer's Disease
por: Chakravarthy, Madhuri, et al.
Publicado: (2017)

The Role of Nucleases and Nucleic Acid Editing Enzymes in the Regulation of Self-Nucleic Acid Sensing
por: Santa, Pauline, et al.
Publicado: (2021)

Selection of a Nuclease-Resistant RNA Aptamer Targeting CD19
por: Esposito, Carla L., et al.
Publicado: (2021)

DNAzymes: Expanding the Potential of Nucleic Acid Therapeutics
por: Larcher, Leon M., et al.
Publicado: (2023)

Corrigendum: The Role of Nucleases and Nucleic Acid Editing Enzymes in the Regulation of Self-Nucleic Acid Sensing
por: Santa, Pauline, et al.
Publicado: (2021)

Construction of a Bivalent Thrombin Binding Aptamer and Its Antidote with Improved Properties
por: Hughes, Quintin W., et al.
Publicado: (2017)

A Locked Nucleic Acid Probe Based on Selective Salt-Induced Effect Detects Single Nucleotide Polymorphisms
por: Zhang, Jing, et al.
Publicado: (2015)

A novel approach for microRNA in situ hybridization using locked nucleic acid probes
por: Paulsen, Isabella W., et al.
Publicado: (2021)

Selective Targeting to Glioma with Nucleic Acid Aptamers
por: Aptekar, Shraddha, et al.
Publicado: (2015)

Aptamers, the Nucleic Acid Antibodies, in Cancer Therapy
por: Fu, Zhaoying, et al.
Publicado: (2020)

Recent Progress and Opportunities for Nucleic Acid Aptamers
por: Byun, Jonghoe
Publicado: (2021)

Cannot write session to /tmp/vufind_sessions/sess_tnuifebgm4fg5g86vjn6km9s0g