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Amniotic Epithelial Cells from the Human Placenta Potently Suppress a Mouse Model of Multiple Sclerosis

Human amniotic epithelial cells (hAEC) have stem cell-like features and immunomodulatory properties. Here we show that hAEC significantly suppressed splenocyte proliferation in vitro and potently attenuated a mouse model of multiple sclerosis (MS). Central nervous system (CNS) CD3(+) T cell and F4/8...

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Autores principales: Liu, Yu Han, Vaghjiani, Vijesh, Tee, Jing Yang, To, Kelly, Cui, Peng, Oh, Ding Yuan, Manuelpillai, Ursula, Toh, Ban-Hock, Chan, James
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3338525/
https://www.ncbi.nlm.nih.gov/pubmed/22563398
http://dx.doi.org/10.1371/journal.pone.0035758
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author Liu, Yu Han
Vaghjiani, Vijesh
Tee, Jing Yang
To, Kelly
Cui, Peng
Oh, Ding Yuan
Manuelpillai, Ursula
Toh, Ban-Hock
Chan, James
author_facet Liu, Yu Han
Vaghjiani, Vijesh
Tee, Jing Yang
To, Kelly
Cui, Peng
Oh, Ding Yuan
Manuelpillai, Ursula
Toh, Ban-Hock
Chan, James
author_sort Liu, Yu Han
collection PubMed
description Human amniotic epithelial cells (hAEC) have stem cell-like features and immunomodulatory properties. Here we show that hAEC significantly suppressed splenocyte proliferation in vitro and potently attenuated a mouse model of multiple sclerosis (MS). Central nervous system (CNS) CD3(+) T cell and F4/80(+) monocyte/macrophage infiltration and demyelination were significantly reduced with hAEC treatment. Besides the known secretion of prostaglandin E2 (PGE2), we report the novel finding that hAEC utilize transforming growth factor-β (TGF-β) for immunosuppression. Neutralization of TGF-β or PGE2 in splenocyte proliferation assays significantly reduced hAEC-induced suppression. Splenocytes from hAEC-treated mice showed a Th2 cytokine shift with significantly elevated IL-5 production. While transferred CFSE-labeled hAEC could be detected in the lung, none were identified in the CNS or in lymphoid organs. This is the first report documenting the therapeutic effect of hAEC in a MS-like model and suggest that hAEC may have potential for use as therapy for MS.
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spelling pubmed-33385252012-05-04 Amniotic Epithelial Cells from the Human Placenta Potently Suppress a Mouse Model of Multiple Sclerosis Liu, Yu Han Vaghjiani, Vijesh Tee, Jing Yang To, Kelly Cui, Peng Oh, Ding Yuan Manuelpillai, Ursula Toh, Ban-Hock Chan, James PLoS One Research Article Human amniotic epithelial cells (hAEC) have stem cell-like features and immunomodulatory properties. Here we show that hAEC significantly suppressed splenocyte proliferation in vitro and potently attenuated a mouse model of multiple sclerosis (MS). Central nervous system (CNS) CD3(+) T cell and F4/80(+) monocyte/macrophage infiltration and demyelination were significantly reduced with hAEC treatment. Besides the known secretion of prostaglandin E2 (PGE2), we report the novel finding that hAEC utilize transforming growth factor-β (TGF-β) for immunosuppression. Neutralization of TGF-β or PGE2 in splenocyte proliferation assays significantly reduced hAEC-induced suppression. Splenocytes from hAEC-treated mice showed a Th2 cytokine shift with significantly elevated IL-5 production. While transferred CFSE-labeled hAEC could be detected in the lung, none were identified in the CNS or in lymphoid organs. This is the first report documenting the therapeutic effect of hAEC in a MS-like model and suggest that hAEC may have potential for use as therapy for MS. Public Library of Science 2012-04-26 /pmc/articles/PMC3338525/ /pubmed/22563398 http://dx.doi.org/10.1371/journal.pone.0035758 Text en Liu et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Liu, Yu Han
Vaghjiani, Vijesh
Tee, Jing Yang
To, Kelly
Cui, Peng
Oh, Ding Yuan
Manuelpillai, Ursula
Toh, Ban-Hock
Chan, James
Amniotic Epithelial Cells from the Human Placenta Potently Suppress a Mouse Model of Multiple Sclerosis
title Amniotic Epithelial Cells from the Human Placenta Potently Suppress a Mouse Model of Multiple Sclerosis
title_full Amniotic Epithelial Cells from the Human Placenta Potently Suppress a Mouse Model of Multiple Sclerosis
title_fullStr Amniotic Epithelial Cells from the Human Placenta Potently Suppress a Mouse Model of Multiple Sclerosis
title_full_unstemmed Amniotic Epithelial Cells from the Human Placenta Potently Suppress a Mouse Model of Multiple Sclerosis
title_short Amniotic Epithelial Cells from the Human Placenta Potently Suppress a Mouse Model of Multiple Sclerosis
title_sort amniotic epithelial cells from the human placenta potently suppress a mouse model of multiple sclerosis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3338525/
https://www.ncbi.nlm.nih.gov/pubmed/22563398
http://dx.doi.org/10.1371/journal.pone.0035758
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