Optimized Expression and Purification for High-Activity Preparations of Algal [FeFe]-Hydrogenase

BACKGROUND: Recombinant expression and purification of metallo-enzymes, including hydrogenases, at high-yields is challenging due to complex, and enzyme specific, post-translational maturation processes. Low fidelities of maturation result in preparations containing a significant fraction of inactiv...

Descripción completa

Detalles Bibliográficos
Autores principales: Yacoby, Iftach, Tegler, Lotta Tollstoy, Pochekailov, Sergii, Zhang, Shuguang, King, Paul W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3338550/
https://www.ncbi.nlm.nih.gov/pubmed/22563413
http://dx.doi.org/10.1371/journal.pone.0035886

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3338550/
https://www.ncbi.nlm.nih.gov/pubmed/22563413
http://dx.doi.org/10.1371/journal.pone.0035886

Ejemplares similares