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Optimized Expression and Purification for High-Activity Preparations of Algal [FeFe]-Hydrogenase

BACKGROUND: Recombinant expression and purification of metallo-enzymes, including hydrogenases, at high-yields is challenging due to complex, and enzyme specific, post-translational maturation processes. Low fidelities of maturation result in preparations containing a significant fraction of inactiv...

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Detalles Bibliográficos
Autores principales:	Yacoby, Iftach, Tegler, Lotta Tollstoy, Pochekailov, Sergii, Zhang, Shuguang, King, Paul W.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2012
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3338550/ https://www.ncbi.nlm.nih.gov/pubmed/22563413 http://dx.doi.org/10.1371/journal.pone.0035886

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