Factors That Affect Large Subunit Ribosomal DNA Amplicon Sequencing Studies of Fungal Communities: Classification Method, Primer Choice, and Error

Nuclear large subunit ribosomal DNA is widely used in fungal phylogenetics and to an increasing extent also amplicon-based environmental sequencing. The relatively short reads produced by next-generation sequencing, however, makes primer choice and sequence error important variables for obtaining ac...

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Detalles Bibliográficos
Autores principales: Porter, Teresita M., Golding, G. Brian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3338786/
https://www.ncbi.nlm.nih.gov/pubmed/22558215
http://dx.doi.org/10.1371/journal.pone.0035749

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3338786/
https://www.ncbi.nlm.nih.gov/pubmed/22558215
http://dx.doi.org/10.1371/journal.pone.0035749

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