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Biochemical, electron microscopic and immunohistological observations of cationic detergent-extracted cells: detection and improved preservation of microextensions and ultramicroextensions

BACKGROUND: Filopodia, retraction fibers and microvilli, are fragile microextensions of the plasma membrane that are easily damaged by mechanical force during specimen preparation for microscopy. To preserve these structures for electron microscopy glutaraldehyde is generally used, but it often caus...

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Detalles Bibliográficos
Autor principal: Nakamura, Fumihiko
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2001
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC33398/
https://www.ncbi.nlm.nih.gov/pubmed/11425343
http://dx.doi.org/10.1186/1471-2121-2-10