Sequencing by Hybridization of Long Targets

Sequencing by Hybridization (SBH) reconstructs an n-long target DNA sequence from its biochemically determined l-long subsequences. In the standard approach, the length of a uniformly random sequence that can be unambiguously reconstructed is limited to [Image: see text] due to repetitive subsequenc...

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Detalles Bibliográficos
Autores principales: Qin, Yu, Schneider, Tobias M., Brenner, Michael P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3344849/
https://www.ncbi.nlm.nih.gov/pubmed/22574124
http://dx.doi.org/10.1371/journal.pone.0035819
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author Qin, Yu
Schneider, Tobias M.
Brenner, Michael P.
author_facet Qin, Yu
Schneider, Tobias M.
Brenner, Michael P.
author_sort Qin, Yu
collection PubMed
description Sequencing by Hybridization (SBH) reconstructs an n-long target DNA sequence from its biochemically determined l-long subsequences. In the standard approach, the length of a uniformly random sequence that can be unambiguously reconstructed is limited to [Image: see text] due to repetitive subsequences causing reconstruction degeneracies. We present a modified sequencing method that overcomes this limitation without the need for different types of biochemical assays and is robust to error.
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spelling pubmed-33448492012-05-09 Sequencing by Hybridization of Long Targets Qin, Yu Schneider, Tobias M. Brenner, Michael P. PLoS One Research Article Sequencing by Hybridization (SBH) reconstructs an n-long target DNA sequence from its biochemically determined l-long subsequences. In the standard approach, the length of a uniformly random sequence that can be unambiguously reconstructed is limited to [Image: see text] due to repetitive subsequences causing reconstruction degeneracies. We present a modified sequencing method that overcomes this limitation without the need for different types of biochemical assays and is robust to error. Public Library of Science 2012-05-04 /pmc/articles/PMC3344849/ /pubmed/22574124 http://dx.doi.org/10.1371/journal.pone.0035819 Text en Qin et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Qin, Yu
Schneider, Tobias M.
Brenner, Michael P.
Sequencing by Hybridization of Long Targets
title Sequencing by Hybridization of Long Targets
title_full Sequencing by Hybridization of Long Targets
title_fullStr Sequencing by Hybridization of Long Targets
title_full_unstemmed Sequencing by Hybridization of Long Targets
title_short Sequencing by Hybridization of Long Targets
title_sort sequencing by hybridization of long targets
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3344849/
https://www.ncbi.nlm.nih.gov/pubmed/22574124
http://dx.doi.org/10.1371/journal.pone.0035819
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