A Simple and Accurate Two-Step Long DNA Sequences Synthesis Strategy to Improve Heterologous Gene Expression in Pichia

In vitro gene chemical synthesis is a powerful tool to improve the expression of gene in heterologous system. In this study, a two-step gene synthesis strategy that combines an assembly PCR and an overlap extension PCR (AOE) was developed. In this strategy, the chemically synthesized oligonucleotide...

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Detalles Bibliográficos
Autores principales: Yang, Jiang-Ke, Chen, Fang-Yuan, Yan, Xiang-Xiang, Miao, Li-Hong, Dai, Jiang-Hong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3344903/
https://www.ncbi.nlm.nih.gov/pubmed/22574193
http://dx.doi.org/10.1371/journal.pone.0036607

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3344903/
https://www.ncbi.nlm.nih.gov/pubmed/22574193
http://dx.doi.org/10.1371/journal.pone.0036607

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