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Screening Estrogenic Activities of Chemicals or Mixtures In Vivo Using Transgenic (cyp19a1b-GFP) Zebrafish Embryos

The tg(cyp19a1b-GFP) transgenic zebrafish expresses GFP (green fluorescent protein) under the control of the cyp19a1b gene, encoding brain aromatase. This gene has two major characteristics: (i) it is only expressed in radial glial progenitors in the brain of fish and (ii) it is exquisitely sensitiv...

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Autores principales: Brion, François, Le Page, Yann, Piccini, Benjamin, Cardoso, Olivier, Tong, Sok-Keng, Chung, Bon-chu, Kah, Olivier
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3346763/
https://www.ncbi.nlm.nih.gov/pubmed/22586461
http://dx.doi.org/10.1371/journal.pone.0036069
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author Brion, François
Le Page, Yann
Piccini, Benjamin
Cardoso, Olivier
Tong, Sok-Keng
Chung, Bon-chu
Kah, Olivier
author_facet Brion, François
Le Page, Yann
Piccini, Benjamin
Cardoso, Olivier
Tong, Sok-Keng
Chung, Bon-chu
Kah, Olivier
author_sort Brion, François
collection PubMed
description The tg(cyp19a1b-GFP) transgenic zebrafish expresses GFP (green fluorescent protein) under the control of the cyp19a1b gene, encoding brain aromatase. This gene has two major characteristics: (i) it is only expressed in radial glial progenitors in the brain of fish and (ii) it is exquisitely sensitive to estrogens. Based on these properties, we demonstrate that natural or synthetic hormones (alone or in binary mixture), including androgens or progestagens, and industrial chemicals induce a concentration-dependent GFP expression in radial glial progenitors. As GFP expression can be quantified by in vivo imaging, this model presents a very powerful tool to screen and characterize compounds potentially acting as estrogen mimics either directly or after metabolization by the zebrafish embryo. This study also shows that radial glial cells that act as stem cells are direct targets for a large panel of endocrine disruptors, calling for more attention regarding the impact of environmental estrogens and/or certain pharmaceuticals on brain development. Altogether these data identify this in vivo bioassay as an interesting alternative to detect estrogen mimics in hazard and risk assessment perspective.
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spelling pubmed-33467632012-05-14 Screening Estrogenic Activities of Chemicals or Mixtures In Vivo Using Transgenic (cyp19a1b-GFP) Zebrafish Embryos Brion, François Le Page, Yann Piccini, Benjamin Cardoso, Olivier Tong, Sok-Keng Chung, Bon-chu Kah, Olivier PLoS One Research Article The tg(cyp19a1b-GFP) transgenic zebrafish expresses GFP (green fluorescent protein) under the control of the cyp19a1b gene, encoding brain aromatase. This gene has two major characteristics: (i) it is only expressed in radial glial progenitors in the brain of fish and (ii) it is exquisitely sensitive to estrogens. Based on these properties, we demonstrate that natural or synthetic hormones (alone or in binary mixture), including androgens or progestagens, and industrial chemicals induce a concentration-dependent GFP expression in radial glial progenitors. As GFP expression can be quantified by in vivo imaging, this model presents a very powerful tool to screen and characterize compounds potentially acting as estrogen mimics either directly or after metabolization by the zebrafish embryo. This study also shows that radial glial cells that act as stem cells are direct targets for a large panel of endocrine disruptors, calling for more attention regarding the impact of environmental estrogens and/or certain pharmaceuticals on brain development. Altogether these data identify this in vivo bioassay as an interesting alternative to detect estrogen mimics in hazard and risk assessment perspective. Public Library of Science 2012-05-07 /pmc/articles/PMC3346763/ /pubmed/22586461 http://dx.doi.org/10.1371/journal.pone.0036069 Text en Brion et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Brion, François
Le Page, Yann
Piccini, Benjamin
Cardoso, Olivier
Tong, Sok-Keng
Chung, Bon-chu
Kah, Olivier
Screening Estrogenic Activities of Chemicals or Mixtures In Vivo Using Transgenic (cyp19a1b-GFP) Zebrafish Embryos
title Screening Estrogenic Activities of Chemicals or Mixtures In Vivo Using Transgenic (cyp19a1b-GFP) Zebrafish Embryos
title_full Screening Estrogenic Activities of Chemicals or Mixtures In Vivo Using Transgenic (cyp19a1b-GFP) Zebrafish Embryos
title_fullStr Screening Estrogenic Activities of Chemicals or Mixtures In Vivo Using Transgenic (cyp19a1b-GFP) Zebrafish Embryos
title_full_unstemmed Screening Estrogenic Activities of Chemicals or Mixtures In Vivo Using Transgenic (cyp19a1b-GFP) Zebrafish Embryos
title_short Screening Estrogenic Activities of Chemicals or Mixtures In Vivo Using Transgenic (cyp19a1b-GFP) Zebrafish Embryos
title_sort screening estrogenic activities of chemicals or mixtures in vivo using transgenic (cyp19a1b-gfp) zebrafish embryos
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3346763/
https://www.ncbi.nlm.nih.gov/pubmed/22586461
http://dx.doi.org/10.1371/journal.pone.0036069
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