Deamination of 5-Methylcytosine Residues in Mammalian Cells

DNA demethylation in mammalia occurs after fertilization and during embryogenesis and accompanies cell aging and cancer transformation. With the help of the primer extension reaction, MALDI MS and DNA cleavage by thymine DNA glycosylase deamination of 5-methylcytosine residues has been shown to take...

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Detalles Bibliográficos
Autores principales: Gromenko, E.V., Spirin, P.V., Kubareva, E.A., Romanova, E.A., Prassolov, V.S., Shpanchenko, O.V., Dontsova, O.A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: A.I. Gordeyev 2009
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3347525/
https://www.ncbi.nlm.nih.gov/pubmed/22649624
Descripción
Sumario:DNA demethylation in mammalia occurs after fertilization and during embryogenesis and accompanies cell aging and cancer transformation. With the help of the primer extension reaction, MALDI MS and DNA cleavage by thymine DNA glycosylase deamination of 5-methylcytosine residues has been shown to take place when the model methylated DNA duplexes are treated with nuclear extracts from the cell lines CHO, HeLa, and Skov3. The hypothesis that deamination of 5-methylcytosine is the first stage of demethylation in mammalia has been postulated.

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