Cargando…

Deamination of 5-Methylcytosine Residues in Mammalian Cells

DNA demethylation in mammalia occurs after fertilization and during embryogenesis and accompanies cell aging and cancer transformation. With the help of the primer extension reaction, MALDI MS and DNA cleavage by thymine DNA glycosylase deamination of 5-methylcytosine residues has been shown to take...

Descripción completa

Detalles Bibliográficos
Autores principales: Gromenko, E.V., Spirin, P.V., Kubareva, E.A., Romanova, E.A., Prassolov, V.S., Shpanchenko, O.V., Dontsova, O.A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: A.I. Gordeyev 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3347525/
https://www.ncbi.nlm.nih.gov/pubmed/22649624
_version_ 1782232285003644928
author Gromenko, E.V.
Spirin, P.V.
Kubareva, E.A.
Romanova, E.A.
Prassolov, V.S.
Shpanchenko, O.V.
Dontsova, O.A.
author_facet Gromenko, E.V.
Spirin, P.V.
Kubareva, E.A.
Romanova, E.A.
Prassolov, V.S.
Shpanchenko, O.V.
Dontsova, O.A.
author_sort Gromenko, E.V.
collection PubMed
description DNA demethylation in mammalia occurs after fertilization and during embryogenesis and accompanies cell aging and cancer transformation. With the help of the primer extension reaction, MALDI MS and DNA cleavage by thymine DNA glycosylase deamination of 5-methylcytosine residues has been shown to take place when the model methylated DNA duplexes are treated with nuclear extracts from the cell lines CHO, HeLa, and Skov3. The hypothesis that deamination of 5-methylcytosine is the first stage of demethylation in mammalia has been postulated.
format Online
Article
Text
id pubmed-3347525
institution National Center for Biotechnology Information
language English
publishDate 2009
publisher A.I. Gordeyev
record_format MEDLINE/PubMed
spelling pubmed-33475252012-05-30 Deamination of 5-Methylcytosine Residues in Mammalian Cells Gromenko, E.V. Spirin, P.V. Kubareva, E.A. Romanova, E.A. Prassolov, V.S. Shpanchenko, O.V. Dontsova, O.A. Acta Naturae Research Article DNA demethylation in mammalia occurs after fertilization and during embryogenesis and accompanies cell aging and cancer transformation. With the help of the primer extension reaction, MALDI MS and DNA cleavage by thymine DNA glycosylase deamination of 5-methylcytosine residues has been shown to take place when the model methylated DNA duplexes are treated with nuclear extracts from the cell lines CHO, HeLa, and Skov3. The hypothesis that deamination of 5-methylcytosine is the first stage of demethylation in mammalia has been postulated. A.I. Gordeyev 2009-10 /pmc/articles/PMC3347525/ /pubmed/22649624 Text en Copyright © 2009 Park-media Ltd. http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Gromenko, E.V.
Spirin, P.V.
Kubareva, E.A.
Romanova, E.A.
Prassolov, V.S.
Shpanchenko, O.V.
Dontsova, O.A.
Deamination of 5-Methylcytosine Residues in Mammalian Cells
title Deamination of 5-Methylcytosine Residues in Mammalian Cells
title_full Deamination of 5-Methylcytosine Residues in Mammalian Cells
title_fullStr Deamination of 5-Methylcytosine Residues in Mammalian Cells
title_full_unstemmed Deamination of 5-Methylcytosine Residues in Mammalian Cells
title_short Deamination of 5-Methylcytosine Residues in Mammalian Cells
title_sort deamination of 5-methylcytosine residues in mammalian cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3347525/
https://www.ncbi.nlm.nih.gov/pubmed/22649624
work_keys_str_mv AT gromenkoev deaminationof5methylcytosineresiduesinmammaliancells
AT spirinpv deaminationof5methylcytosineresiduesinmammaliancells
AT kubarevaea deaminationof5methylcytosineresiduesinmammaliancells
AT romanovaea deaminationof5methylcytosineresiduesinmammaliancells
AT prassolovvs deaminationof5methylcytosineresiduesinmammaliancells
AT shpanchenkoov deaminationof5methylcytosineresiduesinmammaliancells
AT dontsovaoa deaminationof5methylcytosineresiduesinmammaliancells