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Deamination of 5-Methylcytosine Residues in Mammalian Cells
DNA demethylation in mammalia occurs after fertilization and during embryogenesis and accompanies cell aging and cancer transformation. With the help of the primer extension reaction, MALDI MS and DNA cleavage by thymine DNA glycosylase deamination of 5-methylcytosine residues has been shown to take...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
A.I. Gordeyev
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3347525/ https://www.ncbi.nlm.nih.gov/pubmed/22649624 |
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author | Gromenko, E.V. Spirin, P.V. Kubareva, E.A. Romanova, E.A. Prassolov, V.S. Shpanchenko, O.V. Dontsova, O.A. |
author_facet | Gromenko, E.V. Spirin, P.V. Kubareva, E.A. Romanova, E.A. Prassolov, V.S. Shpanchenko, O.V. Dontsova, O.A. |
author_sort | Gromenko, E.V. |
collection | PubMed |
description | DNA demethylation in mammalia occurs after fertilization and during embryogenesis and accompanies cell aging and cancer transformation. With the help of the primer extension reaction, MALDI MS and DNA cleavage by thymine DNA glycosylase deamination of 5-methylcytosine residues has been shown to take place when the model methylated DNA duplexes are treated with nuclear extracts from the cell lines CHO, HeLa, and Skov3. The hypothesis that deamination of 5-methylcytosine is the first stage of demethylation in mammalia has been postulated. |
format | Online Article Text |
id | pubmed-3347525 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | A.I. Gordeyev |
record_format | MEDLINE/PubMed |
spelling | pubmed-33475252012-05-30 Deamination of 5-Methylcytosine Residues in Mammalian Cells Gromenko, E.V. Spirin, P.V. Kubareva, E.A. Romanova, E.A. Prassolov, V.S. Shpanchenko, O.V. Dontsova, O.A. Acta Naturae Research Article DNA demethylation in mammalia occurs after fertilization and during embryogenesis and accompanies cell aging and cancer transformation. With the help of the primer extension reaction, MALDI MS and DNA cleavage by thymine DNA glycosylase deamination of 5-methylcytosine residues has been shown to take place when the model methylated DNA duplexes are treated with nuclear extracts from the cell lines CHO, HeLa, and Skov3. The hypothesis that deamination of 5-methylcytosine is the first stage of demethylation in mammalia has been postulated. A.I. Gordeyev 2009-10 /pmc/articles/PMC3347525/ /pubmed/22649624 Text en Copyright © 2009 Park-media Ltd. http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Gromenko, E.V. Spirin, P.V. Kubareva, E.A. Romanova, E.A. Prassolov, V.S. Shpanchenko, O.V. Dontsova, O.A. Deamination of 5-Methylcytosine Residues in Mammalian Cells |
title | Deamination of 5-Methylcytosine Residues in Mammalian Cells |
title_full | Deamination of 5-Methylcytosine Residues in Mammalian Cells |
title_fullStr | Deamination of 5-Methylcytosine Residues in Mammalian Cells |
title_full_unstemmed | Deamination of 5-Methylcytosine Residues in Mammalian Cells |
title_short | Deamination of 5-Methylcytosine Residues in Mammalian Cells |
title_sort | deamination of 5-methylcytosine residues in mammalian cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3347525/ https://www.ncbi.nlm.nih.gov/pubmed/22649624 |
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