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A Real Time Metridia Luciferase Based Non-Invasive Reporter Assay of Mammalian Cell Viability and Cytotoxicity via the β-actin Promoter and Enhancer

Secreted reporter molecules offer a means to evaluate biological processes in real time without the need to sacrifice samples at pre-determined endpoints. Here we have adapted the secreted bioluminescent reporter gene, Metridia luciferase, for use in a real-time viability assay for mammalian cells....

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Detalles Bibliográficos
Autores principales: Lupold, Shawn E., Johnson, Tamara, Chowdhury, Wasim H., Rodriguez, Ronald
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3348918/
https://www.ncbi.nlm.nih.gov/pubmed/22590558
http://dx.doi.org/10.1371/journal.pone.0036535