Efficient RNA interference in patients' acute lymphoblastic leukemia cells amplified as xenografts in mice

BACKGROUND: Signaling studies in cell lines are hampered by non-physiological alterations obtained in vitro. Physiologic primary tumor cells from patients with leukemia require passaging through immune-compromised mice for amplification. The aim was to enable molecular work in patients' ALL cel...

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Detalles Bibliográficos
Autores principales: Höfig, Ines, Ehrhardt, Harald, Jeremias, Irmela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Methodology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3349556/
https://www.ncbi.nlm.nih.gov/pubmed/22448764
http://dx.doi.org/10.1186/1478-811X-10-8
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author Höfig, Ines
Ehrhardt, Harald
Jeremias, Irmela
author_facet Höfig, Ines
Ehrhardt, Harald
Jeremias, Irmela
author_sort Höfig, Ines
collection PubMed
description BACKGROUND: Signaling studies in cell lines are hampered by non-physiological alterations obtained in vitro. Physiologic primary tumor cells from patients with leukemia require passaging through immune-compromised mice for amplification. The aim was to enable molecular work in patients' ALL cells by establishing siRNA transfection into cells amplified in mice. RESULTS: We established delivering siRNA into these cells without affecting cell viability. Knockdown of single or multiple genes reduced constitutive or induced protein expression accompanied by marked signaling alterations. CONCLUSION: Our novel technique allows using patient-derived tumor cells instead of cell lines for signaling studies in leukemia.
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spelling pubmed-33495562012-05-11 Efficient RNA interference in patients' acute lymphoblastic leukemia cells amplified as xenografts in mice Höfig, Ines Ehrhardt, Harald Jeremias, Irmela Cell Commun Signal Methodology BACKGROUND: Signaling studies in cell lines are hampered by non-physiological alterations obtained in vitro. Physiologic primary tumor cells from patients with leukemia require passaging through immune-compromised mice for amplification. The aim was to enable molecular work in patients' ALL cells by establishing siRNA transfection into cells amplified in mice. RESULTS: We established delivering siRNA into these cells without affecting cell viability. Knockdown of single or multiple genes reduced constitutive or induced protein expression accompanied by marked signaling alterations. CONCLUSION: Our novel technique allows using patient-derived tumor cells instead of cell lines for signaling studies in leukemia. BioMed Central 2012-03-26 /pmc/articles/PMC3349556/ /pubmed/22448764 http://dx.doi.org/10.1186/1478-811X-10-8 Text en Copyright ©2012 Höfig et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Methodology
Höfig, Ines
Ehrhardt, Harald
Jeremias, Irmela
Efficient RNA interference in patients' acute lymphoblastic leukemia cells amplified as xenografts in mice
title Efficient RNA interference in patients' acute lymphoblastic leukemia cells amplified as xenografts in mice
title_full Efficient RNA interference in patients' acute lymphoblastic leukemia cells amplified as xenografts in mice
title_fullStr Efficient RNA interference in patients' acute lymphoblastic leukemia cells amplified as xenografts in mice
title_full_unstemmed Efficient RNA interference in patients' acute lymphoblastic leukemia cells amplified as xenografts in mice
title_short Efficient RNA interference in patients' acute lymphoblastic leukemia cells amplified as xenografts in mice
title_sort efficient rna interference in patients' acute lymphoblastic leukemia cells amplified as xenografts in mice
topic Methodology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3349556/
https://www.ncbi.nlm.nih.gov/pubmed/22448764
http://dx.doi.org/10.1186/1478-811X-10-8
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