A novel PCR-based method for high throughput prokaryotic expression of antimicrobial peptide genes

BACKGROUND: To facilitate the screening of large quantities of new antimicrobial peptides (AMPs), we describe a cost-effective method for high throughput prokaryotic expression of AMPs. EDDIE, an autoproteolytic mutant of the N-terminal autoprotease, Npro, from classical swine fever virus, was selec...

Descripción completa

Detalles Bibliográficos
Autores principales: Ke, Tao, Liang, Su, Huang, Jin, Mao, Han, Chen, Jibao, Dong, Caihua, Huang, Junyan, Liu, Shengyi, Kang, Jianxiong, Liu, Dongqi, Ma, Xiangdong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3350388/
https://www.ncbi.nlm.nih.gov/pubmed/22439858
http://dx.doi.org/10.1186/1472-6750-12-10

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3350388/
https://www.ncbi.nlm.nih.gov/pubmed/22439858
http://dx.doi.org/10.1186/1472-6750-12-10

Ejemplares similares