Sample richness and genetic diversity as drivers of chimera formation in nSSU metagenetic analyses

Eukaryotic diversity in environmental samples is often assessed via PCR-based amplification of nSSU genes. However, estimates of diversity derived from pyrosequencing environmental data sets are often inflated, mainly because of the formation of chimeric sequences during PCR amplification. Chimeras...

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Detalles Bibliográficos
Autores principales: Fonseca, V. G., Nichols, B., Lallias, D., Quince, C., Carvalho, G. R., Power, D. M., Creer, S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2012
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3351157/
https://www.ncbi.nlm.nih.gov/pubmed/22278883
http://dx.doi.org/10.1093/nar/gks002

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3351157/
https://www.ncbi.nlm.nih.gov/pubmed/22278883
http://dx.doi.org/10.1093/nar/gks002

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