Cargando…
Sample richness and genetic diversity as drivers of chimera formation in nSSU metagenetic analyses
Eukaryotic diversity in environmental samples is often assessed via PCR-based amplification of nSSU genes. However, estimates of diversity derived from pyrosequencing environmental data sets are often inflated, mainly because of the formation of chimeric sequences during PCR amplification. Chimeras...
| Autores principales: | , , , , , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2012
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3351157/ https://www.ncbi.nlm.nih.gov/pubmed/22278883 http://dx.doi.org/10.1093/nar/gks002 |