A transposase strategy for creating libraries of circularly permuted proteins

A simple approach for creating libraries of circularly permuted proteins is described that is called PERMutation Using Transposase Engineering (PERMUTE). In PERMUTE, the transposase MuA is used to randomly insert a minitransposon that can function as a protein expression vector into a plasmid that c...

Descripción completa

Detalles Bibliográficos
Autores principales: Mehta, Manan M., Liu, Shirley, Silberg, Jonathan J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2012
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3351165/
https://www.ncbi.nlm.nih.gov/pubmed/22319214
http://dx.doi.org/10.1093/nar/gks060

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3351165/
https://www.ncbi.nlm.nih.gov/pubmed/22319214
http://dx.doi.org/10.1093/nar/gks060

Ejemplares similares