Stable Gene Targeting in Human Cells Using Single-Strand Oligonucleotides with Modified Bases

Recent advances allow multiplexed genome engineering in E. coli, employing easily designed oligonucleotides to edit multiple loci simultaneously. A similar technology in human cells would greatly expedite functional genomics, both by enhancing our ability to test how individual variants such as sing...

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Detalles Bibliográficos
Autores principales: Rios, Xavier, Briggs, Adrian W., Christodoulou, Danos, Gorham, Josh M., Seidman, Jonathan G., Church, George M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3351460/
https://www.ncbi.nlm.nih.gov/pubmed/22615794
http://dx.doi.org/10.1371/journal.pone.0036697

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3351460/
https://www.ncbi.nlm.nih.gov/pubmed/22615794
http://dx.doi.org/10.1371/journal.pone.0036697

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