Cargando…

Stable Gene Targeting in Human Cells Using Single-Strand Oligonucleotides with Modified Bases

Recent advances allow multiplexed genome engineering in E. coli, employing easily designed oligonucleotides to edit multiple loci simultaneously. A similar technology in human cells would greatly expedite functional genomics, both by enhancing our ability to test how individual variants such as sing...

Descripción completa

Detalles Bibliográficos
Autores principales:	Rios, Xavier, Briggs, Adrian W., Christodoulou, Danos, Gorham, Josh M., Seidman, Jonathan G., Church, George M.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2012
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3351460/ https://www.ncbi.nlm.nih.gov/pubmed/22615794 http://dx.doi.org/10.1371/journal.pone.0036697

Ejemplares similares

Stable transmission of targeted gene modification using single-stranded oligonucleotides with flanking LNAs
por: Andrieu-Soler, Charlotte, et al.
Publicado: (2005)

Mechanisms of single-stranded phosphorothioate modified antisense oligonucleotide accumulation in hepatocytes
por: Koller, Erich, et al.
Publicado: (2011)

AAV Recombineering with Single Strand Oligonucleotides
por: Hirsch, Matthew L., et al.
Publicado: (2009)

Enhanced gene repair mediated by methyl-CpG-modified single-stranded oligonucleotides
por: Bertoni, Carmen, et al.
Publicado: (2009)

Monitoring integrity and localization of modified single-stranded RNA oligonucleotides using ultrasensitive fluorescence methods
por: Heissig, Philipp, et al.
Publicado: (2017)

The involvement of replication in single stranded oligonucleotide-mediated gene repair
por: Huen, Michael S. Y., et al.
Publicado: (2006)

Development and antitumor activity of a BCL-2 targeted single-stranded DNA oligonucleotide
por: Rodrigueza, Wendi V., et al.
Publicado: (2014)

Strand bias influences the mechanism of gene editing directed by single-stranded DNA oligonucleotides
por: Falgowski, Kerry, et al.
Publicado: (2011)

Reduction of gene repair by selenomethionine with the use of single-stranded oligonucleotides
por: Schwartz, Timothy R, et al.
Publicado: (2007)

RecA-independent single-stranded DNA oligonucleotide-mediated mutagenesis
por: Murphy, Kenan C, et al.
Publicado: (2010)

Methods for the Preparation of Large Quantities of Complex Single-Stranded Oligonucleotide Libraries
por: Murgha, Yusuf E., et al.
Publicado: (2014)

Natural polyphenol assisted delivery of single-strand oligonucleotides by cationic polymers
por: Shen, Wanwan, et al.
Publicado: (2020)

Single-Stranded Oligonucleotide-Mediated Inhibition of Respiratory Syncytial Virus Infection
por: Pålsson, Sandra Axberg, et al.
Publicado: (2020)

Comparative Study of Single-stranded Oligonucleotides Secondary Structure Prediction Tools
por: Binet, Thomas, et al.
Publicado: (2023)

Single-stranded oligonucleotide-mediated in vivo gene repair in the rd1 retina
por: Andrieu-Soler, Charlotte, et al.
Publicado: (2007)

Cleavage of Oligonucleotides Containing a P3’→N5’ Phosphoramidate Linkage Mediated by Single-Stranded Oligonucleotide Templates
por: Ito, Kosuke Ramon, et al.
Publicado: (2011)

Simple and Rapid Enzymatic Method for the Synthesis of Single-Strand Oligonucleotides Containing Trifluorothymidine
por: Suzuki, Norihiko, et al.
Publicado: (2010)

Clinical Applications of Single-Stranded Oligonucleotides: Current Landscape of Approved and In-Development Therapeutics
por: Scharner, Juergen, et al.
Publicado: (2021)

Starting and stopping RNA polymerase III transcription on single-stranded DNA oligonucleotides
por: Lama, Lodoe, et al.
Publicado: (2022)

Mismatched single stranded antisense oligonucleotides can induce efficient dystrophin splice switching
por: Fragall, Clayton T, et al.
Publicado: (2011)

A Suite of Therapeutically-Inspired Nucleic Acid Logic Systems for Conditional Generation of Single-Stranded and Double-Stranded Oligonucleotides
por: Zakrevsky, Paul, et al.
Publicado: (2019)

Modified bases enable high-efficiency oligonucleotide-mediated allelic replacement via mismatch repair evasion
por: Wang, Harris H., et al.
Publicado: (2011)

Assessing single-stranded oligonucleotide drug-induced effects in vitro reveals key risk factors for thrombocytopenia
por: Sewing, Sabine, et al.
Publicado: (2017)

SIDT2 mediates gymnosis, the uptake of naked single-stranded oligonucleotides into living cells
por: Takahashi, Masayuki, et al.
Publicado: (2017)

Single Stranded Fully Modified-Phosphorothioate Oligonucleotides can Induce Structured Nuclear Inclusions, Alter Nuclear Protein Localization and Disturb the Transcriptome In Vitro
por: Flynn, Loren L., et al.
Publicado: (2022)

Simultaneous targeted exchange of two nucleotides by single-stranded oligonucleotides clusters within a region of about fourteen nucleotides
por: Hegele, Heike, et al.
Publicado: (2008)

Natural and Modified Oligonucleotide Sequences Show Distinct Strand Displacement Kinetics and These Are Affected Further by Molecular Crowders
por: Domljanovic, Ivana, et al.
Publicado: (2022)

Furan-modified oligonucleotides for fast, high-yielding and site-selective DNA inter-strand cross-linking with non-modified complements(†)
por: Stevens, Kristof, et al.
Publicado: (2009)

Systematic Screening of Commonly Used Commercial Transfection Reagents towards Efficient Transfection of Single-Stranded Oligonucleotides
por: Wang, Tao, et al.
Publicado: (2018)

Liver-Targeted Anti-HBV Single-Stranded Oligonucleotides with Locked Nucleic Acid Potently Reduce HBV Gene Expression In Vivo
por: Javanbakht, Hassan, et al.
Publicado: (2018)

Regulation of Gene Editing Activity Directed by Single-Stranded Oligonucleotides and CRISPR/Cas9 Systems
por: Bialk, Pawel, et al.
Publicado: (2015)

Long-adapter single-strand oligonucleotide probes for the massively multiplexed cloning of kilobase genome regions
por: Tosi, Lorenzo, et al.
Publicado: (2017)

AptaMat: a matrix-based algorithm to compare single-stranded oligonucleotides secondary structures
por: Binet, Thomas, et al.
Publicado: (2022)

Rapid Synthesis of a Long Double-Stranded Oligonucleotide from a Single-Stranded Nucleotide Using Magnetic Beads and an Oligo Library
por: Pengpumkiat, Sumate, et al.
Publicado: (2016)

Direct Mutagenesis of Thousands of Genomic Targets Using Microarray-Derived Oligonucleotides
por: Bonde, Mads T., et al.
Publicado: (2014)

Triplex-forming oligonucleotides: a third strand for DNA nanotechnology
por: Chandrasekaran, Arun Richard, et al.
Publicado: (2018)

Guidelines for Experiments Using Antisense Oligonucleotides and Double-Stranded RNAs
por: Gagnon, Keith T., et al.
Publicado: (2019)

Scalable amplification of strand subsets from chip-synthesized oligonucleotide libraries
por: Schmidt, Thorsten L., et al.
Publicado: (2015)

Use of Synthetic Single-Stranded Oligonucleotides as Artificial Test Soiling for Validation of Surgical Instrument Cleaning Processes
por: Wilhelm, Nadja, et al.
Publicado: (2014)

From the Cover: The Minipig is a Suitable Non-Rodent Model in the Safety Assessment of Single Stranded Oligonucleotides
por: Braendli-Baiocco, Annamaria, et al.
Publicado: (2017)

Cannot write session to /tmp/vufind_sessions/sess_tj0qe4ecehvbuf5pcv8vitn918