A modular and optimized single marker system for generating Trypanosoma brucei cell lines expressing T7 RNA polymerase and the tetracycline repressor

Here, we present a simple modular extendable vector system for introducing the T7 RNA polymerase and tetracycline repressor genes into Trypanosoma brucei. This novel system exploits developments in our understanding of gene expression and genome organization to produce a streamlined plasmid optimize...

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Detalles Bibliográficos
Autores principales: Poon, S. K., Peacock, L., Gibson, W., Gull, K., Kelly, S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society 2012
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3352093/
https://www.ncbi.nlm.nih.gov/pubmed/22645659
http://dx.doi.org/10.1098/rsob.110037

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3352093/
https://www.ncbi.nlm.nih.gov/pubmed/22645659
http://dx.doi.org/10.1098/rsob.110037

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