Improved methods for detection of β-galactosidase (lacZ) activity in hard tissue

The β-galactosidase gene (lacZ) of Escherichia coli is widely used as a reporter gene. The expression of lacZ can be detected by enzyme-based histochemical staining using chromogenic substrates such as 5-bromo-4-chloro-3-indolyl-β-d-galactoside (X-gal). Because the enzymatic activity of lacZ is vuln...

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Detalles Bibliográficos
Autores principales: Shimada, Akemi, Komatsu, Koichiro, Nakashima, Kazuhisa, Pöschl, Ernst, Nifuji, Akira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer-Verlag 2012
Materias:
Short Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3353101/
https://www.ncbi.nlm.nih.gov/pubmed/22371055
http://dx.doi.org/10.1007/s00418-012-0936-1

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3353101/
https://www.ncbi.nlm.nih.gov/pubmed/22371055
http://dx.doi.org/10.1007/s00418-012-0936-1

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