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Improved methods for detection of β-galactosidase (lacZ) activity in hard tissue
The β-galactosidase gene (lacZ) of Escherichia coli is widely used as a reporter gene. The expression of lacZ can be detected by enzyme-based histochemical staining using chromogenic substrates such as 5-bromo-4-chloro-3-indolyl-β-d-galactoside (X-gal). Because the enzymatic activity of lacZ is vuln...
Autores principales: | Shimada, Akemi, Komatsu, Koichiro, Nakashima, Kazuhisa, Pöschl, Ernst, Nifuji, Akira |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer-Verlag
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3353101/ https://www.ncbi.nlm.nih.gov/pubmed/22371055 http://dx.doi.org/10.1007/s00418-012-0936-1 |
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