Crystal Structure of a Complex of DNA with One AT-Hook of HMGA1

We present here for the first time the crystal structure of an AT-hook domain. We show the structure of an AT-hook of the ubiquitous nuclear protein HMGA1, combined with the oligonucleotide d(CGAATTAATTCG)(2), which has two potential AATT interacting groups. Interaction with only one of them is foun...

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Detalles Bibliográficos
Autores principales: Fonfría-Subirós, Elsa, Acosta-Reyes, Francisco, Saperas, Núria, Pous, Joan, Subirana, Juan A., Campos, J. Lourdes
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3353895/
https://www.ncbi.nlm.nih.gov/pubmed/22615915
http://dx.doi.org/10.1371/journal.pone.0037120
Descripción
Sumario:We present here for the first time the crystal structure of an AT-hook domain. We show the structure of an AT-hook of the ubiquitous nuclear protein HMGA1, combined with the oligonucleotide d(CGAATTAATTCG)(2), which has two potential AATT interacting groups. Interaction with only one of them is found. The structure presents analogies and significant differences with previous NMR studies: the AT-hook forms hydrogen bonds between main-chain NH groups and thymines in the minor groove, DNA is bent and the minor groove is widened.

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