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Expression of an Epitope-Tagged Virulence Protein in Rickettsia parkeri Using Transposon Insertion

Despite recent advances in our ability to genetically manipulate Rickettsia, little has been done to employ genetic tools to study the expression and localization of Rickettsia virulence proteins. Using a mariner-based Himar1 transposition system, we expressed an epitope-tagged variant of the actin...

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Detalles Bibliográficos
Autores principales: Welch, Matthew D., Reed, Shawna C. O., Lamason, Rebecca L., Serio, Alisa W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3356282/
https://www.ncbi.nlm.nih.gov/pubmed/22624012
http://dx.doi.org/10.1371/journal.pone.0037310