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Expression of an Epitope-Tagged Virulence Protein in Rickettsia parkeri Using Transposon Insertion
Despite recent advances in our ability to genetically manipulate Rickettsia, little has been done to employ genetic tools to study the expression and localization of Rickettsia virulence proteins. Using a mariner-based Himar1 transposition system, we expressed an epitope-tagged variant of the actin...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2012
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3356282/ https://www.ncbi.nlm.nih.gov/pubmed/22624012 http://dx.doi.org/10.1371/journal.pone.0037310 |