An Optimized Real-Time PCR to Avoid Species-/Tissue-Associated Inhibition for H5N1 Detection in Ferret and Monkey Tissues

The real-time PCR diagnostics for avian influenza virus H5N1 in tissue specimens are often suboptimal, since naturally occurring PCR inhibitors present in samples, or unanticipated match of primer to unsequenced species' genome. With the principal aim of optimizing the SYBR Green real-time PCR...

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Detalles Bibliográficos
Autores principales: Zhan, LingJun, Bao, LinLin, Li, FengDi, Lv, Qi, Xu, LiLi, Qin, Chuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Scientific World Journal 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3356745/
https://www.ncbi.nlm.nih.gov/pubmed/22645461
http://dx.doi.org/10.1100/2012/907095

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3356745/
https://www.ncbi.nlm.nih.gov/pubmed/22645461
http://dx.doi.org/10.1100/2012/907095

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