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A Predictive Model of Intein Insertion Site for Use in the Engineering of Molecular Switches
Inteins are intervening protein domains with self-splicing ability that can be used as molecular switches to control activity of their host protein. Successfully engineering an intein into a host protein requires identifying an insertion site that permits intein insertion and splicing while allowing...
Autores principales: | Apgar, James, Ross, Mary, Zuo, Xiao, Dohle, Sarah, Sturtevant, Derek, Shen, Binzhang, de la Vega, Humberto, Lessard, Philip, Lazar, Gabor, Raab, R. Michael |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3359363/ https://www.ncbi.nlm.nih.gov/pubmed/22649521 http://dx.doi.org/10.1371/journal.pone.0037355 |
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