Cargando…

Improved Somatic Mutagenesis in Zebrafish Using Transcription Activator-Like Effector Nucleases (TALENs)

Zinc Finger Nucleases (ZFNs) made by Context-Dependent Assembly (CoDA) and Transcription Activator-Like Effector Nucleases (TALENs) provide robust and user-friendly technologies for efficiently inactivating genes in zebrafish. These designer nucleases bind to and cleave DNA at particular target site...

Descripción completa

Detalles Bibliográficos
Autores principales:	Moore, Finola E., Reyon, Deepak, Sander, Jeffry D., Martinez, Sarah A., Blackburn, Jessica S., Khayter, Cyd, Ramirez, Cherie L., Joung, J. Keith, Langenau, David M.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2012
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3360007/ https://www.ncbi.nlm.nih.gov/pubmed/22655075 http://dx.doi.org/10.1371/journal.pone.0037877

Ejemplares similares

Targeted gene disruption in somatic zebrafish cells using engineered TALENs
por: Sander, Jeffry D., et al.
Publicado: (2011)

High frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells
por: Fu, Yanfang, et al.
Publicado: (2013)

FLASH Assembly of TALENs Enables High-Throughput Genome Editing
por: Reyon, Deepak, et al.
Publicado: (2012)

Highly efficient generation of heritable zebrafish gene mutations using homo- and heterodimeric TALENs
por: Cade, Lindsay, et al.
Publicado: (2012)

Broad Specificity Profiling of TALENs Results in Engineered Nucleases With Improved DNA Cleavage Specificity
por: Guilinger, John P., et al.
Publicado: (2014)

Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing
por: Tsai, Shengdar Q., et al.
Publicado: (2014)

Improving CRISPR-Cas nuclease specificity using truncated guide RNAs
por: Fu, Yanfang, et al.
Publicado: (2014)

Rapid Mutation of Endogenous Zebrafish Genes Using Zinc Finger Nucleases Made by Oligomerized Pool ENgineering (OPEN)
por: Foley, Jonathan E., et al.
Publicado: (2009)

Selection-Free Zinc-Finger Nuclease Engineering by Context-Dependent Assembly (CoDA)
por: Sander, Jeffry D., et al.
Publicado: (2010)

In silico abstraction of zinc finger nuclease cleavage profiles reveals an expanded landscape of off-target sites
por: Sander, Jeffry D., et al.
Publicado: (2013)

ZFNGenome: A comprehensive resource for locating zinc finger nuclease target sites in model organisms
por: Reyon, Deepak, et al.
Publicado: (2011)

Efficient In Vivo Genome Editing Using RNA-Guided Nucleases
por: Hwang, Woong Y., et al.
Publicado: (2013)

Targeting Human MicroRNA Genes Using Engineered Tal-Effector Nucleases (TALENs)
por: Hu, Ruozhen, et al.
Publicado: (2013)

Targeted Deletion and Inversion of Tandemly Arrayed Genes in Arabidopsis thaliana Using Zinc Finger Nucleases
por: Qi, Yiping, et al.
Publicado: (2013)

Generation of gene disruptions by transcription activator-like effector nucleases (TALENs) in Xenopus tropicalis embryos
por: Lei, Yong, et al.
Publicado: (2013)

Transcription Activator-Like Effector Nucleases (TALEN)-Mediated Targeted DNA Insertion in Potato Plants
por: Forsyth, Adrienne, et al.
Publicado: (2016)

Evaluation of OPEN Zinc Finger Nucleases for Direct Gene Targeting of the ROSA26 Locus in Mouse Embryos
por: Hermann, Mario, et al.
Publicado: (2012)

A One-Step System for Convenient and Flexible Assembly of Transcription Activator-Like Effector Nucleases (TALENs)
por: Zhao, Jinlong, et al.
Publicado: (2016)

Through the Looking Glass: Visualizing Leukemia Growth, Migration, and Engraftment Using Fluorescent Transgenic Zebrafish
por: Moore, Finola E., et al.
Publicado: (2012)

Targeted Mutagenesis of Arabidopsis thaliana Using Engineered TAL Effector Nucleases
por: Christian, Michelle, et al.
Publicado: (2013)

Targeted gene disruption by use of transcription activator-like effector nuclease (TALEN) in the water flea Daphnia pulex
por: Hiruta, Chizue, et al.
Publicado: (2014)

GUIDE-Seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases
por: Tsai, Shengdar Q., et al.
Publicado: (2014)

Establishment of pten knockout medaka with transcription activator–like effector nucleases (TALENs) as a model of PTEN deficiency disease
por: Matsuzaki, Yuriko, et al.
Publicado: (2017)

Genome editing of oncogenes with ZFNs and TALENs: caveats in nuclease design
por: Shankar, Sumitra, et al.
Publicado: (2018)

Heritable and Precise Zebrafish Genome Editing Using a CRISPR-Cas System
por: Hwang, Woong Y., et al.
Publicado: (2013)

Development of zebrafish medulloblastoma-like PNET model by TALEN-mediated somatic gene inactivation
por: Shim, Jaegal, et al.
Publicado: (2017)

An optimized TALEN application for mutagenesis and screening in Drosophila melanogaster
por: Lee, Han B, et al.
Publicado: (2015)

Expanded activity of dimer nucleases by combining ZFN and TALEN for genome editing
por: Yan, Wei, et al.
Publicado: (2013)

mRNA transfection of a novel TAL effector nuclease (TALEN) facilitates efficient knockout of HIV co-receptor CCR5
por: Mock, Ulrike, et al.
Publicado: (2015)

Continuous directed evolution of DNA-binding proteins to improve TALEN specificity
por: Hubbard, Basil P., et al.
Publicado: (2015)

The I-TevI Nuclease and Linker Domains Contribute to the Specificity of Monomeric TALENs
por: Kleinstiver, Benjamin P., et al.
Publicado: (2014)

ZiFiT (Zinc Finger Targeter): an updated zinc finger engineering tool
por: Sander, Jeffry D., et al.
Publicado: (2010)

Rapid tumor induction in zebrafish by TALEN-mediated somatic inactivation of the retinoblastoma1 tumor suppressor rb1
por: Solin, Staci L., et al.
Publicado: (2015)

Zebrafish as a model to assess cancer heterogeneity, progression and relapse
por: Blackburn, Jessica S., et al.
Publicado: (2014)

Robust, synergistic regulation of human gene expression using TALE activators
por: Maeder, Morgan L., et al.
Publicado: (2013)

Context influences on TALE–DNA binding revealed by quantitative profiling
por: Rogers, Julia M., et al.
Publicado: (2015)

Activities and specificities of CRISPR/Cas9 and Cas12a nucleases for targeted mutagenesis in maize
por: Lee, Keunsub, et al.
Publicado: (2018)

Revealing Off-Target Cleavage Specificities of Zinc Finger Nucleases by In Vitro Selection
por: Pattanayak, Vikram, et al.
Publicado: (2011)

Optimized cell transplantation using adult rag2 mutant zebrafish
por: Tang, Qin, et al.
Publicado: (2014)

Targeted Mutagenesis in the Malaria Mosquito Using TALE Nucleases
por: Smidler, Andrea L., et al.
Publicado: (2013)

Cannot write session to /tmp/vufind_sessions/sess_ti8c4o95tutqchg2r12emf9qhr