siRNA off-target effects in genome-wide screens identify signaling pathway members

We introduce a method for analyzing small interfering RNA (siRNA) genetic screens based entirely on off-target effects. Using a screen for members of the Wnt pathway, we demonstrate that this method identifies known pathway components, some of which are not present in the screening library. This tec...

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Detalles Bibliográficos
Autores principales: Buehler, Eugen, Khan, Aly A., Marine, Shane, Rajaram, Misha, Bahl, Amit, Burchard, Julja, Ferrer, Marc
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3361704/
https://www.ncbi.nlm.nih.gov/pubmed/22645644
http://dx.doi.org/10.1038/srep00428

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3361704/
https://www.ncbi.nlm.nih.gov/pubmed/22645644
http://dx.doi.org/10.1038/srep00428

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