siRNA off-target effects in genome-wide screens identify signaling pathway members

We introduce a method for analyzing small interfering RNA (siRNA) genetic screens based entirely on off-target effects. Using a screen for members of the Wnt pathway, we demonstrate that this method identifies known pathway components, some of which are not present in the screening library. This tec...

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Detalles Bibliográficos
Autores principales: Buehler, Eugen, Khan, Aly A., Marine, Shane, Rajaram, Misha, Bahl, Amit, Burchard, Julja, Ferrer, Marc
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3361704/
https://www.ncbi.nlm.nih.gov/pubmed/22645644
http://dx.doi.org/10.1038/srep00428
Descripción
Sumario:We introduce a method for analyzing small interfering RNA (siRNA) genetic screens based entirely on off-target effects. Using a screen for members of the Wnt pathway, we demonstrate that this method identifies known pathway components, some of which are not present in the screening library. This technique can be applied to siRNA screen results retroactively to confirm positives and identify genes missed using conventional methods for on-target gene selection.

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