siRNA off-target effects in genome-wide screens identify signaling pathway members

We introduce a method for analyzing small interfering RNA (siRNA) genetic screens based entirely on off-target effects. Using a screen for members of the Wnt pathway, we demonstrate that this method identifies known pathway components, some of which are not present in the screening library. This tec...

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Detalles Bibliográficos
Autores principales: Buehler, Eugen, Khan, Aly A., Marine, Shane, Rajaram, Misha, Bahl, Amit, Burchard, Julja, Ferrer, Marc
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3361704/
https://www.ncbi.nlm.nih.gov/pubmed/22645644
http://dx.doi.org/10.1038/srep00428
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author Buehler, Eugen
Khan, Aly A.
Marine, Shane
Rajaram, Misha
Bahl, Amit
Burchard, Julja
Ferrer, Marc
author_facet Buehler, Eugen
Khan, Aly A.
Marine, Shane
Rajaram, Misha
Bahl, Amit
Burchard, Julja
Ferrer, Marc
author_sort Buehler, Eugen
collection PubMed
description We introduce a method for analyzing small interfering RNA (siRNA) genetic screens based entirely on off-target effects. Using a screen for members of the Wnt pathway, we demonstrate that this method identifies known pathway components, some of which are not present in the screening library. This technique can be applied to siRNA screen results retroactively to confirm positives and identify genes missed using conventional methods for on-target gene selection.
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spelling pubmed-33617042012-05-29 siRNA off-target effects in genome-wide screens identify signaling pathway members Buehler, Eugen Khan, Aly A. Marine, Shane Rajaram, Misha Bahl, Amit Burchard, Julja Ferrer, Marc Sci Rep Article We introduce a method for analyzing small interfering RNA (siRNA) genetic screens based entirely on off-target effects. Using a screen for members of the Wnt pathway, we demonstrate that this method identifies known pathway components, some of which are not present in the screening library. This technique can be applied to siRNA screen results retroactively to confirm positives and identify genes missed using conventional methods for on-target gene selection. Nature Publishing Group 2012-05-29 /pmc/articles/PMC3361704/ /pubmed/22645644 http://dx.doi.org/10.1038/srep00428 Text en Copyright © 2012, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-sa/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-ShareALike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/
spellingShingle Article
Buehler, Eugen
Khan, Aly A.
Marine, Shane
Rajaram, Misha
Bahl, Amit
Burchard, Julja
Ferrer, Marc
siRNA off-target effects in genome-wide screens identify signaling pathway members
title siRNA off-target effects in genome-wide screens identify signaling pathway members
title_full siRNA off-target effects in genome-wide screens identify signaling pathway members
title_fullStr siRNA off-target effects in genome-wide screens identify signaling pathway members
title_full_unstemmed siRNA off-target effects in genome-wide screens identify signaling pathway members
title_short siRNA off-target effects in genome-wide screens identify signaling pathway members
title_sort sirna off-target effects in genome-wide screens identify signaling pathway members
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3361704/
https://www.ncbi.nlm.nih.gov/pubmed/22645644
http://dx.doi.org/10.1038/srep00428
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