Cargando…
Flexibility in MuA Transposase Family Protein Structures: Functional Mapping with Scanning Mutagenesis and Sequence Alignment of Protein Homologues
MuA transposase protein is a member of the retroviral integrase superfamily (RISF). It catalyzes DNA cleavage and joining reactions via an initial assembly and subsequent structural transitions of a protein-DNA complex, known as the Mu transpososome, ultimately attaching transposon DNA to non-specif...
Autores principales: | Rasila, Tiina S., Vihinen, Mauno, Paulin, Lars, Haapa-Paananen, Saija, Savilahti, Harri |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3362531/ https://www.ncbi.nlm.nih.gov/pubmed/22666413 http://dx.doi.org/10.1371/journal.pone.0037922 |
Ejemplares similares
-
Mu transpososome activity-profiling yields hyperactive MuA variants for highly efficient genetic and genome engineering
por: Rasila, Tiina S, et al.
Publicado: (2018) -
Characteristics of MuA transposase-catalyzed processing of model transposon end DNA hairpin substrates
por: Saariaho, Anna-Helena, et al.
Publicado: (2006) -
Universal platform for quantitative analysis of DNA transposition
por: Pajunen, Maria I, et al.
Publicado: (2010) -
An assay to monitor the activity of DNA transposition complexes yields a general quality control measure for transpositional recombination reactions
por: Pulkkinen, Elsi, et al.
Publicado: (2014) -
High-precision mapping of protein–protein interfaces: an integrated genetic strategy combining en masse mutagenesis and DNA-level parallel analysis on a yeast two-hybrid platform
por: Pajunen, Maria, et al.
Publicado: (2007)