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Comparative Analysis of Gene Expression Level by Quantitative Real-Time PCR Has Limited Application in Objects with Different Morphology

qRT-PCR is a generally acknowledged method for gene expression analysis due to its precision and reproducibility. However, it is well known that the accuracy of qRT-PCR data varies greatly depending on the experimental design and data analysis. Recently, a set of guidelines has been proposed that ai...

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Detalles Bibliográficos
Autores principales: Demidenko, Natalia V., Penin, Aleksey A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3364230/
https://www.ncbi.nlm.nih.gov/pubmed/22666472
http://dx.doi.org/10.1371/journal.pone.0038161