Thermostable DNA Polymerase from a Viral Metagenome Is a Potent RT-PCR Enzyme

Viral metagenomic libraries are a promising but previously untapped source of new reagent enzymes. Deep sequencing and functional screening of viral metagenomic DNA from a near-boiling thermal pool identified clones expressing thermostable DNA polymerase (Pol) activity. Among these, 3173 Pol demonst...

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Detalles Bibliográficos
Autores principales: Moser, Michael J., DiFrancesco, Robert A., Gowda, Krishne, Klingele, Audrey J., Sugar, Darby R., Stocki, Stacy, Mead, David A., Schoenfeld, Thomas W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3366922/
https://www.ncbi.nlm.nih.gov/pubmed/22675552
http://dx.doi.org/10.1371/journal.pone.0038371

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3366922/
https://www.ncbi.nlm.nih.gov/pubmed/22675552
http://dx.doi.org/10.1371/journal.pone.0038371

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