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Thermostable DNA Polymerase from a Viral Metagenome Is a Potent RT-PCR Enzyme

Viral metagenomic libraries are a promising but previously untapped source of new reagent enzymes. Deep sequencing and functional screening of viral metagenomic DNA from a near-boiling thermal pool identified clones expressing thermostable DNA polymerase (Pol) activity. Among these, 3173 Pol demonst...

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Detalles Bibliográficos
Autores principales:	Moser, Michael J., DiFrancesco, Robert A., Gowda, Krishne, Klingele, Audrey J., Sugar, Darby R., Stocki, Stacy, Mead, David A., Schoenfeld, Thomas W.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2012
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3366922/ https://www.ncbi.nlm.nih.gov/pubmed/22675552 http://dx.doi.org/10.1371/journal.pone.0038371

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