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Impact of wavefront distortion and scattering on 2-photon microscopy in mammalian brain tissue

Two-photon (2P) microscopy is widely used in neuroscience, but the optical properties of brain tissue are poorly understood. We have investigated the effect of brain tissue on the 2P point spread function (PSF(2P)) by imaging fluorescent beads through living cortical slices. By combining this with m...

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Detalles Bibliográficos
Autores principales: Chaigneau, Emmanuelle, Wright, Amanda J., Poland, Simon P., Girkin, John M., Silver, R. Angus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Optical Society of America 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3369558/
https://www.ncbi.nlm.nih.gov/pubmed/22109156
http://dx.doi.org/10.1364/OE.19.022755