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A Rapid Cloning Method Employing Orthogonal End Protection
We describe a novel in vitro cloning strategy that combines standard tools in molecular biology with a basic protecting group concept to create a versatile framework for the rapid and seamless assembly of modular DNA building blocks into functional open reading frames. Analogous to chemical synthesi...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2012
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3369885/ https://www.ncbi.nlm.nih.gov/pubmed/22685543 http://dx.doi.org/10.1371/journal.pone.0037617 |