The Duration of Antigen-Stimulation Significantly Alters the Diversity of Multifunctional CD4 T Cells Measured by Intracellular Cytokine Staining

The assessment of antigen-specific T cell responses by intracellular cytokine staining (ICS) has become a routine technique in studies of vaccination and immunity. Here, we highlight how the duration of in vitro antigen pre-stimulation, combined with the cytokine accumulation period, are critical pa...

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Detalles Bibliográficos
Autores principales: Kaveh, Daryan A., Whelan, Adam O., Hogarth, Philip J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3373578/
https://www.ncbi.nlm.nih.gov/pubmed/22719990
http://dx.doi.org/10.1371/journal.pone.0038926

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3373578/
https://www.ncbi.nlm.nih.gov/pubmed/22719990
http://dx.doi.org/10.1371/journal.pone.0038926

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