Generation of Virus-Free Induced Pluripotent Stem Cell Clones on a Synthetic Matrix via a Single Cell Subcloning in the Naïve State

CD34+ cord blood cells can be reprogrammed effectively on dishes coated with a synthetic RGD motif polymer (PronectinF®) using a temperature sensitive Sendai virus vector (SeV TS7) carrying reprogramming factors OCT3/4, SOX2, KLF4 and c-MYC. Dish-shaped human ES cell-like colonies emerged in serum-f...

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Detalles Bibliográficos
Autores principales: Nishishita, Naoki, Shikamura, Masayuki, Takenaka, Chiemi, Takada, Nozomi, Fusak, Noemi, Kawamata, Shin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3374798/
https://www.ncbi.nlm.nih.gov/pubmed/22719883
http://dx.doi.org/10.1371/journal.pone.0038389

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3374798/
https://www.ncbi.nlm.nih.gov/pubmed/22719883
http://dx.doi.org/10.1371/journal.pone.0038389

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