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FRET-Based Localization of Fluorescent Protein Insertions Within the Ryanodine Receptor Type 1

Fluorescent protein (FP) insertions have often been used to localize primary structure elements in mid-resolution 3D cryo electron microscopic (EM) maps of large protein complexes. However, little is known as to the precise spatial relationship between the location of the fused FP and its insertion...

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Detalles Bibliográficos
Autores principales: Raina, Shweta A., Tsai, Jeffrey, Samsó, Montserrat, Fessenden, James D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3374828/
https://www.ncbi.nlm.nih.gov/pubmed/22719904
http://dx.doi.org/10.1371/journal.pone.0038594