Cargando…
FRET-Based Localization of Fluorescent Protein Insertions Within the Ryanodine Receptor Type 1
Fluorescent protein (FP) insertions have often been used to localize primary structure elements in mid-resolution 3D cryo electron microscopic (EM) maps of large protein complexes. However, little is known as to the precise spatial relationship between the location of the fused FP and its insertion...
Autores principales: | Raina, Shweta A., Tsai, Jeffrey, Samsó, Montserrat, Fessenden, James D. |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3374828/ https://www.ncbi.nlm.nih.gov/pubmed/22719904 http://dx.doi.org/10.1371/journal.pone.0038594 |
Ejemplares similares
-
Ca(2+) inactivation of the mammalian ryanodine receptor type 1 in a lipidic environment revealed by cryo-EM
por: Nayak, Ashok R, et al.
Publicado: (2022) -
Three-Dimensional Location of the Imperatoxin a Binding Site on the Ryanodine Receptor
por: Samsó, Montserrat, et al.
Publicado: (1999) -
Site-Specific Labeling of the Type 1 Ryanodine Receptor Using Biarsenical Fluorophores Targeted to Engineered Tetracysteine Motifs
por: Fessenden, James D., et al.
Publicado: (2013) -
Förster Resonance Energy Transfer Measurements of Ryanodine Receptor Type 1 Structure Using a Novel Site-Specific Labeling Method
por: Fessenden, James D.
Publicado: (2009) -
Molecular mechanism of the severe MH/CCD mutation Y522S in skeletal ryanodine receptor (RyR1) by cryo-EM
por: Iyer, Kavita A., et al.
Publicado: (2022)