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Detection of Microregional Hypoxia in Mouse Cerebral Cortex by Two-photon Imaging of Endogenous NADH Fluorescence
The brain's ability to function at high levels of metabolic demand depends on continuous oxygen supply through blood flow and tissue oxygen diffusion. Here we present a visualized experimental and methodological protocol to directly visualize microregional tissue hypoxia and to infer perivascul...
Autores principales: | Polesskaya, Oksana, Sun, Anita, Salahura, Gheorghe, Silva, Jharon N., Dewhurst, Stephen, Kasischke, Karl |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MyJove Corporation
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3376929/ https://www.ncbi.nlm.nih.gov/pubmed/22370971 http://dx.doi.org/10.3791/3466 |
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